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Fluorescence (RFU) 485

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nm ex. M) in SH-SY5Y cells treated with H202 MSE and MIT with or without anti-oxidant NAC (added at 30 minutes). The fluorescence kratom fst tincture dosage athol product DCF was kratom bali 15x extrakt cashiers

measured at 485 nm ex. Premium Thai Kratom Powdered/leaf Pullman the result was generated from a single preliminary experiment.

Arochlor 1254 rat liver S9-mix was used as the exogenous metabolising system and was prepared freshly on the day of the assay. The S9-mix was prepared by mixing 1 part of S9 with 9 parts of co-factor (5. M NADP (Na2) and 27.

For each sample 10000 or 30000 events were collected and aggregated cells were gated out Premium Thai Kratom Powdered/leaf Pullman of the analysis. The percentage of cells at different phases of the cell cycle was determined using ModFit LT MAC 3. CellQuest pro software. PI was

excited at 488 nm using an Argon laser and the fluorescence analysed at 620 nm. Immunoblot For this experiment the procedure was adapted from Laemmli method (Laemmli 1970). SH-SY5Y cells were used as it was the most sensitive cell line for the toxicity effects of MSE and MIT.

Antracyclines

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induce calpaindependanttitin proteolysis and necrosis in capsuleiomyocytes. Genetic toxicity assessment: Employing the best science for human safety evaluation Part IV: A strategy in genotoxicity testing in drug development: Some examples. Toxicological Sciences 98:39-42 Lu W. Models of reactive oxygen species in cancer.

Introduction to toxicology. Taylor and Francis publisher. Effects of Mitragynine on cAMP formation mediated by delta-opiate receptors in NG108-15 Cells. Effect of mitragynine derived from Thai folk medicine on gastric acid secretion through opioid receptor in anesthetized rats.

C (5% CO2) for the designated time period. The adherent cells (HEK 293 and SH-SY5Y cells) were harvested trypsinised and centrifuged as per routine procedures described in chapter 2 sections 2. After this incubation the cells were harvested as previously described (section 2.

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